Journal: Nature Cell Biology
Article Title: The Vps13-like protein BLTP2 regulates phosphatidylethanolamine levels to maintain plasma membrane fluidity and breast cancer aggressiveness
doi: 10.1038/s41556-025-01672-3
Figure Lengend Snippet: a , A plot of BLTP2 mRNA expression score and survival score for ~1,000 cancer cell lines with a deletion of BLTP2 . Data from DepMap. b , Crystal violet-stained colony forming units of HeLa cells plated at the cell number indicated on the top (left). Growth rate of WT and BLTP2 -KO cells ( n = 3) in DMEM with cFBS (right); mean cell number ± s.e.m.; P values from unpaired two-tailed t -tests. c , The AlphaFold predicted secondary structure of yeast Fmp27 (green) and human BLTP2 (magenta) compared and aligned using the Matchmaker plugin of UCSF Chimera. Scale bar, 30 Å. d , Serial dilutions of yeast strains spotted on SC without EtN supplementation and grown for 3 days. e , Western blot (left) of genomically expressed Fmp27-3xHA and Por1 (control). Cells were grown in SC and Fmp27-3xHA and Por1 were immunoprecipitated. Quantification of Fmp27–GFP normalized to Por1 (right). The results are shown as a box–violin plot (the solid bar shows the median and the dotted bar the quartiles); P values from unpaired two-tailed t -tests, n = 3. f , A schematic of Kennedy and CDP-DAG pathways. Created with BioRender.com . g , Serial dilutions yeast strains were spotted on SC without or with 4 mM Cho or 4 mM EtN and grown at 18 °C for 3 days (top). Doubling time of strains grown at 18 °C, with or without EtN supplementation, in a 96-well plate; histograms show mean ± s.e.m. ( n = 3); P values from unpaired two-tailed t -tests (bottom). h , The relative amount of the five major PLs in cells grown at 18 °C and labelled to steady-state with [ 3 H]palmitate; mean ± s.e.m. ( n = 3), P values from two-way ANOVA. i , Serial dilution of yeast strains spotted on SC medium without or with Cho, EtN or PpN and incubated at 23 °C for 3 days. j , The structure of l -[3- 3 H]serine; T indicates the position of tritium (top) and a scheme of PS, PE and PC production by exogenous [ 3 H]serine (top). The bar graph shows the relative abundance of radiolabelled PLs after growth for 1 h at 18 °C in SC (bottom); mean ± s.e.m. ( n = 3); P values from two-way ANOVA. k , The chemical structures of EtN and PpN.
Article Snippet: The Ftractin-EGFP construct was a gift from Dr. Dyche Mullins at UCSF (Addgene plasmid 58473) and MDA-MB-231 cells were a gift from Dr. Rolf Brekken (UT Southwestern Medical Center).
Techniques: Expressing, Staining, Two Tailed Test, Western Blot, Control, Immunoprecipitation, Serial Dilution, Incubation
Addgene inc is a verified supplier 
![a , A plot of BLTP2 mRNA expression score and survival score for ~1,000 cancer cell lines with a deletion of BLTP2 . Data from DepMap. b , Crystal violet-stained colony forming units of HeLa cells plated at the cell number indicated on the top (left). Growth rate of WT and BLTP2 -KO cells ( n = 3) in DMEM with cFBS (right); mean cell number ± s.e.m.; P values from unpaired two-tailed t -tests. c , The AlphaFold predicted secondary structure of yeast Fmp27 (green) and human BLTP2 (magenta) compared and aligned using the Matchmaker plugin of <t>UCSF</t> Chimera. Scale bar, 30 Å. d , Serial dilutions of yeast strains spotted on SC without EtN supplementation and grown for 3 days. e , Western blot (left) of genomically expressed Fmp27-3xHA and Por1 (control). Cells were grown in SC and Fmp27-3xHA and Por1 were immunoprecipitated. Quantification of Fmp27–GFP normalized to Por1 (right). The results are shown as a box–violin plot (the solid bar shows the median and the dotted bar the quartiles); P values from unpaired two-tailed t -tests, n = 3. f , A schematic of Kennedy and CDP-DAG pathways. Created with BioRender.com . g , Serial dilutions yeast strains were spotted on SC without or with 4 mM Cho or 4 mM EtN and grown at 18 °C for 3 days (top). Doubling time of strains grown at 18 °C, with or without EtN supplementation, in a 96-well plate; histograms show mean ± s.e.m. ( n = 3); P values from unpaired two-tailed t -tests (bottom). h , The relative amount of the five major PLs in cells grown at 18 °C and labelled to steady-state with [ 3 H]palmitate; mean ± s.e.m. ( n = 3), P values from two-way ANOVA. i , Serial dilution of yeast strains spotted on SC medium without or with Cho, EtN or PpN and incubated at 23 °C for 3 days. j , The structure of l -[3- 3 H]serine; T indicates the position of tritium (top) and a scheme of PS, PE and PC production by exogenous [ 3 H]serine (top). The bar graph shows the relative abundance of radiolabelled PLs after growth for 1 h at 18 °C in SC (bottom); mean ± s.e.m. ( n = 3); P values from two-way ANOVA. k , The chemical structures of EtN and PpN.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_0902/pmc12270902/pmc12270902__41556_2025_1672_Fig1_HTML.jpg)